The aim of this study is to investigate strength and water purification characteristics of effective microorganism-applied volcanic ash block using flexural strength test and water quality analysis. The specimens were prepared with volcanic ash from Mt. Baekdusan and Mt. Hallasan, and cement as the ratios of 3.5:1, 4.0:1, 4.5:1, 5.0:1 with and without metakaolin. Flexural strength degraded with increasing of the amount of volcanic ash, and increased with addition of metakaolin as a binder. Based on these results, the optimal ratio for fabricating volcanic ash-cement mixture block is determined as 3.5:1 with metakaolin. Furthermore, from water quality analysis on contaminated water, removal ability of effective microorganism-applied volcanic ash-cement mixture block and caged volcanic ash block against T-N, T-P and SS was highly evaluated because of adsorption due to the large specific surface area of volcanic ash. Hence, volcanic ash-cement mixture block and caged volcanic ash block possibly contribute to water purification.