To improve the physical properties, such as swelling and flexibility, of the cotton fiber, sodium hydroxide or cellulase was used for pretreatment before the beating process. Titanium dioxide was blended during beating process to improve the fibrillation of cotton fibers and even distribution of fillers to cotton fibers. Blending with titanium dioxide during beating process, led to improve the tensile strength and beating degree This treatment also improved the opacity, resulted by well dispersed titanium dioxide during blending. By the blending of titanium dioxide during beating process, similar impact of cotton fiber with cellulase or sodium hydroxide pretreatment was achieved. To improve the tensile strength and opacity of cotton paper simultaneously, titanium dioxide blending in during beating process was found as effective treatment.