The process of electromembrane transformation of L-lysine monohydrochlorides into their zwitterionic form in four- and two-chamber electrodialysis apparatus was investigated. The process of transformation at various concentrations of lysine monohydrochloride (0.1-0.6 mol.L-1) was studied and it was established that at the optimum density of current optimal concentrations of lysine hydrochloride during electrodyalisis was in the range of 0.2-0.4 mol.L-1. It was determined that the process of total transformation was accomplished when pH of the lysine solution achieved 10. Changes of concentrations of $Cl^-$ ions and lysine diffused into the neighboring chamber were determined depending on the time. The method developed by us allows adjusting the removal coefficient of $Cl^-$ ions during transformation to a maximal value, the losses of lysine diffused into the next chamber after its return to the technological cycle being less than 1.0 %. The specific energy consumption during the process of transformation in two- and four-chamber electrodialyzers was 0.19 and 0.205 A.h.kg-1 and the current efficiency was 75.9 and 73.1 %, correspondingly. Study of the process of electromembrane transformation allowed obtaining zwitterionic form of L-lysine from L-lysine monohydrochloride with minimal reagent and energy consumption.