Purpose: The purpose of this study was to investigate attitudes and preventive practice of pressure ulcer among hospital nurses and identify factors affecting their preventive practice of pressure ulcer. Methods: Data were collected at April, 2011 in a university hospital in Korea. A total of 140 hospital nurses completed a questionnaire including attitudes and preventive practice of pressure ulcer. Data were analyzed with descriptive statistics, Spearmans rho, and stepwise multiple regression via SPSS/WIN 20.0. Results: Levels of attitudes and preventive practice of pressure ulcer were average 3.65 out of 5 and 2.19 out of 3 respectively. There was significant difference in preventive practice of pressure ulcer by area of practice ($x^2$= 43.35, p<.001), frequency of pressure ulcer care ($x^2$=10.72, p=.013), and experience of wound care education (Ζ=4.73, p=.030). The attitudes toward pressure ulcer prevention were positive correlated to preventive practice among nurses (rho=.190, p=.049). Area of practice, attitude toward pressure ulcer prevention and experience of wound care education explained 48% of variance in preventive practice of pressure ulcer. Conclusion: For improving quality of care, it is important to provide educations to change nurses attitudes toward preventive practice of pressure ulcer.