The ship radiated noise level fluctuates by the interference between direct and reflected paths. The effect of sea surface reflection path on interference depends strongly on sea surface roughness. This paper describes error characteristics of ship acoustic signature estimation by sea surface scattering effect. The coherent reflection coefficient which explains a magnitude of sea surface scattering and its resultant interference acoustic field is analyzed quantitatively as a function of a grazing angle, effective surface height, frequency, source-receiver range and depths of source and receiver. Theoretical interference acoustic field is compared with experimental result for two different sea surfaces and five different frequencies by changing source-receiver range. It is found that both matches well each other and a magnitude of interference acoustic field is decreasing by increasing a grazing angle, effective surface height, frequency, and depths of source and receiver and decreasing source-receiver range. For given experimental conditions, the transmission anomaly which is a bias error of ship acoustic signature estimation, is about a range of 1~3 dB. The bias error of an existing ship radiated noise measurement system is also analyzed considering wind speed, source depth and frequency.