Nutritional composition is the main consideration in the safety assessment of foods derived from genetically modified (GM) crops. In this study, the key nutrients in drought-tolerant rice that had been generated by the insertion of AtCYP78A7 encoding cytochrome P450 protein were analyzed. Results were compared with those obtained from its non-transgenic counterpart and other commercial rice. When transgenic rice lines 10B-5 and 18A-4 were compared with the non-transgenic counterpart, no significant differences were found in their contents of proximates, amino acids, fatty acids, minerals, and vitamins. Except for fiber contents and levels of vitamin $B_2$, most of the measured values fit within the reference ranges established for other commercial rice. These results indicate that the key nutritional composition of drought-tolerant transgenic rice is substantially equivalent to its non-transgenic counterpart. Therefore, insertion of AtCYP78A7 to improve drought tolerance does not change the constitution and quality of key nutrients in brown rice.