Bumblebees are widely used to pollinate various greenhouse crops. Among the different bumblebee species, Bombus ignitus is indigenous to Korea, China, Japan and Russia. B. ignitus undergoes one generation per year, and artificial hibernation is essential for year-round rearing of the bumblebee. Keeping the queens under low-temperature conditions for several months is an effective method for terminating their diapause and promoting colony development. In the present study, we investigated how cold temperature affects the artificial hibernation of B. ignitus queens. Under chilling temperatures of $-2.5^{circ}C$, $0^{circ}C$, $2.5^{circ}C$ and $5^{circ}C$ with constant humidity >80%, the queens stored at $2.5^{circ}C$ exhibited the highest survival rates, which were 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months and 24.0% at 6 months. Lower survival rates were observed at $0^{circ}C$, $5^{circ}C$, $7.5^{circ}C$ and $12.5^{circ}C$. At $2.5^{circ}C$ the colony developmental characteristics after diapause were 1.2- to 1.5-fold greater than those when queens were stored at $5^{circ}C$. Thus, $2.5^{circ}C$ and 70% R.H. were the most favorable chilling temperature and humidity conditions for terminating the diapause of B. ignitus queens.