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The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice
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  • The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice
  • The balneotherapy effect of hydrogen reduced water on UVB-mediated skin injury in hairless mice
저자명
Ignacio. Rosa Mistica,Yoon. Yang-Suk,Sajo. Ma. Easter Joy,Kim. Cheol-Su,Kim. Dong-Heui,Kim. Soo-Ki,Lee. Kyu-Jae
간행물명
Molecular & cellular toxicology
권/호정보
2013년|9권 1호|pp.15-21 (7 pages)
발행정보
대한독성유전단백체학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Exposure to UVB radiation induced skin damage that results to increase risk of skin cancer. Despite the clinical importance of skin-induced damage, antioxidants imposed limited therapeutic success. Hydrogen molecule ($H_2$) has been known as a safe antioxidant in the prevention and therapeutic approach towards several diseases. Drinking hydrogen reduced water (HRW), inhalation of hydrogen gas, and injecting $H_2$-dissolved saline are widely accepted to incorporate $H_2$ in the body. However, there is no document about the beneficial effect of hydrogen water bath. Here, we investigated the effect of hydrogen bathing on the UVB-induced skin damage in hairless mice. For this, mice of the bathing group are allowed to freely swim on HRW, and let the HRW penetrate for 60 mins. Scoring of skin injury, reactive oxygen species (ROS) enzyme activity quantification, cytokine analysis, and ultrastructural change of corneocytes were measured after exposure to UVB radiation of $360-540mJ/cm^2$. In summary, the bathing with HRW significantly reduced the levels of skin damage, as well as increased activity of glutathione peroxidase. Further, the effect of HRW on cytokine network in the skin after UVB exposure revealed that HRW significantly decreased the level of inflammatory cytokines such as IL-$1{eta}$, IL-6, TNF-${alpha}$ and IFN-${gamma}$. Finally, scanning electron microscopy data revealed low number of defected corneocytes and ultrastructural changes, suggesting that HRW bathing would protect UV-induced cell damage.