This study estimated the changes in delays and speeds of vehicles in exclusive bus lane and road when the High Occupancy Vehicles(HOV) use the median bus lane. Synchro simulation tool was used to optimize the traffic signal time on the network and VISSIM was applied to simulate various scenarios. Here, drivers behavior parameters in VISSIM was optimized using Simultaneous Perturbation Stochastic Approximation(SPSA) algorithm in order to represent real traffic condition. Based on the simulation results, the delay in Doan daero was decreased when the volume of HOV in current condition runs on the median bus lane, whereas delay in Doan dongro was increased in all scenarios. The changes in bus speed was not sharply decreased for both study sites, even though the number of HOV increased to 10%. Thus, it could be allowed that the HOV use the median bus lane in Doan dongro and Doan daero. Future research tasks include studying about changes in delay when the HOV use the curb bus lane.