This paper presents an experimental study on the safety of a valve for a special gas cylinder. The test valves that were randomly dismantled from the special gas cylinder were experimented on the gas leakage and operation safeties. The crack, wear and deformation of the valve body, screw thread, safety disk, vent hole, stem and handle components that may affect to the gas leakage safety of a used valve were not found in this experimental study. A painted handle of a valve was partly stripped from the coated surface, and the surface of PT screw of a used valve body was rusted. But, these paint and rust problems do not affected to the gas leakage safety of used valves. And there was no gas leakage in the dismantled valve, and the permanent deformation and partial scars of a valve stem and O-rings were observed on the rubbing surfaces. Thus, the valve seat and O-rings are recommended to be replaced for a gas leakage safety of a dismantled valve. And it is necessary to repair and inspect handle fastening forces for a safe opening and closing operations of a valve.