Objective: The aim of this study is to investigate the gender-differences in vibrotactile responses(sensitivity and displeasure) of residual forearm simulated by vibration stimulation in upper limb(trans-radial) amputees. Background: Several studies have reported that vibration stimulation using the haptic vibrator is one the most effective methods for delivering sensation to an amputees. However, few studies have reported the perception to haptic vibratory stimulus, particularly sensitivity and displeasure. Method: We set up a custom-made vibration stimulation system that included 6 actuators(3 medial parts and 3 lateral parts) and a graphical user interface(GUI)-based acquisition system to investigate changes in residual somatosensory sensibility and displeasure in the forearm of upper limb(trans-radial) amputees. Vibration actuators were attached at the 25%-point on the proximal forearm. Stimulation with 32Hz, 64Hz, or 149Hz of frequency was used for the sensitivity tests and with 32~257Hz of frequency was used for the discomfort experiments. The subjective responses were evaluated on a 10 point scale. Results: The results showed that vibrotactile sensory perception in male amputees were higher than that in female amputees. In male amputees, the response at lateral area of forearm was the most sensitive than medial area; but, female amputees showed similar sensitive areas. Subjects did not experience any discomfort during vibrotactile stimuli. Conclusion: Vibrotactile response in the amputees was dependent on gender as well as area stimulated by vibration. Application: The results might contribute to develop the vibrotactile feedback system for the amputees.