충남 금산군과 전남 완도군, 제주도 송악산 등지에서 서식하고 있는 야생화들로부터 Rhodosporidium fluviale 201-C-1과 Kluyveromyces thermotolerans 150-JE-2(1), Rodosporidium paludigenum과 Candida sp. 80-J-3등의 국내 미기록 효모들을 분리, 선발하여 이들의 미생물학적 특성들을 조사하였다. Rhodosporidium fluviale 201-C-1은 내염성의 무포자 효모로 의균사를 형성하였고 urease 활성을 갖고 있었다. Kluyveromyces thermotolerans 150-JE-2(1)는 내당성과 내염성을 가진 무포자효모로서 $30^{circ}C$, pH 4.0~6.0에서 잘 생육하였다. Candida sp. 80-J-3과 Rhodosporidium paludigenum 86-J-1 균주들은 모두 무성생식을 하는 무포자 효모들이었고 YPD 배지, Vitamin-free 배지와 5% NaCl을 함유한 YPD 배지 등에서 잘 생육하였다. 또한 Candida sp. 80-J-3 균주의 urease 활성은 음성이었으나 Rhodosporidium paludigenum 86-J-1 균주는 urease 활성이 양성이면서 pH 7.0~8.0에서 잘 생육하였다.
Rhodosporidium fluviale 201-C-1 and Kluyveromyces thermotolerans 150-JE-2(1) were isolated from wild flowers in Geumsan-gun, Chungcheongnam-do and Wando-gun, Jeollanam-do, respectively. Rhodosporidium paludigenum 86-J-1 and Candida sp. 80-J-3 were also isolated from flowers in Jeju island, Korea. Characteristics for these yeasts were not reported before. Therefore, morphological and physiological characteristics for these yeasts were investigated in this study. R. fluviale 201-C-1 was halophilic asporogenous yeast and formed pseudomycelium. It also grew at $30^{circ}C$ and pH 4.0-6.0. K. thermotolerans 150-JE-2(1) was sugar-tolerant and halotolerant asporogenous yeast. It formed pseudomycelium and showed positive urease activity. Candida sp. 80-J-3 and R. paludigenum 86-J-1 were asporogenous yeasts, and grew well in yeast extract-peptone dextrose(YPD) medium, potato-dextrose medium as well as vitamin-free medium and YPD medium containing 5% NaCl.
