In order to evaluate fatigue endurance for an MTB(mountain bike) frame, FEM(finite element method) analysis was performed. For evaluating the fatigue endurance of the MTB frame, the S-N data for Al-6061 fillet weldment were compared with the stress analysis results through FEM analysis of the frame. Three loading condition, pedalling, horizontal and vertical loading conditions were considered for fatigue endurance evaluation. Horizontal loading(+1200 N) condition was found to be the most severe to the frame. The maximum von Mises stress of the frame under horizontal loading(+1200 N) condition was determined 294 MPa through FEM analysis of the frame. Conclusively, on the basis of fatigue strength of 200 MPa at the number of cycles of 50,000, the MTB frame has an improper safety factor of approximately 0.25, suggesting that this frame needs reinforcement.