Heating wire cutting type separation mechanism has been widely used for cube satellite applications due to its design constraints such as small size of $10cm{ imes}10cm{ imes}10cm$ and light weight of less than 1kg. In addition, usage of pyro technic device is not allowed for cube satellite application. The conventional methods have some disadvantages of relatively small mechanical constraint force and the system complexity for the multi-deployable systems. In this paper, a separation nut type non-explosive separation mechanism has been proposed and investigated. The effectiveness of the design has been verified through the qualification tests of the mechanism.