This study was evaluated that seasonal change of the hesperidin content was in relation to the skin color and weight of fruit in outer or inner side of the canopy, for determine the optimum time and the index of most abundant hesperidin content in lemon fruits juice. The hesperidin content was different with fruit growth and on fruit bearing site of the tree. The fruits of the either side contained the highest hesperidin value at 162 and 176 days after anthesis just after de-greening and the value was highest in the fruits of outer side of the canopy. This time also corresponded to the time just before the cessation of fruit growth. These results suggested that the optimum harvesting period for more hesperidin content was the stage just after de-greening and just before fruit growth cessation.