In this study, paper mill applicability of two kinds of cotton pulps which have different initial freeness, fiber length and intrinsic fiber strength were investigated. Basic properties such as CED viscosity, fiber length, and crystallinity of major two kinds of cotton pulps were analyzed, and beatability of cotton pulps and physical properties of handsheet made from two kinds of cotton pulps were compared. Laboratory beating was performed at different refining conditions such as refining loads and freenesses. Relationship between beating degree and physical properties of handsheet were compared to seek optimum condition of refining for different cotton pulps application to paper mill.