Objectives : The purpose of the study is to investigate the relationship between self-leadership in clinical practice and stress in dental hygiene majoring students. Methods : Subjects were 250 dental hygiene majoring students in J area from March 20 to April 20, 2012. Data were analyzed using the statistical package SPSS WIN 12.0 for frequency, mean and standard deviation analysis, one-way ANOVA and multiple regression. Results : There were significant differences between Satisfaction and clinical practice, practice and major stress factors(p<0.01). There were significant differences between practices, satisfaction, and self-expectations(p<0.001). The explanatory power of the model was 9%(p<0.05). The combination of self-leadership, activity, interpersonal factors were very important factors and the explanatory power of the model was 8%(p<0.001). Conclusions : Self-leadership is able to decrease stress. Self-leadership is very important in clinical practice in dental hygiene majoring students.