In order to study the effect of organic fertilizer on dry matter (DM) yield, nitrogen fixation and transfer from pea to barley, an experiment was carried out from May to June in 2008 in Incheon. A completely randomized block design with three replications was used for the experiment and one reference plot assigned each treatment for nitrogen fixation evaluation. Seeding mixture was 40kg barley and 80kg pea per ha. N rate of 40, 80 and 120kg/ha as organic fertilizer was applied at seeding. The equivalent of 1kg per ha as $(15NH_4)_2SO_4$ Solution at 99.8 atom N was applied to the plots ($30{ imes}20cm$) at mid April. Forage was harvested from each plot in ripening stage at ground level and separated into barley and pea. Nitrogen fixation was 32.4%, 23.4% and 0% at three different organic N levels. Transfer rate were from 47.6% to 21.8% in difference method and 24.6% and 21.4% on $^{15}N$ dilution method. N Transfer amount were from 92.8kg/ha to 41.9kg/ha on difference method and 47.3kg in the 40kg N plots and 49.7kg in the 80kg N plots on $^{15}N$ dilution method but there was no N transfer in 120kg N organic fertilizer plots. Benefit from increased organic fertilizer was not clear in terms of nitrogen fixation and transfer from pea to barley in barley and pea mixtures.