Ventilation efficiency has an important role in agricultural facilities such as greenhouse and livestock house to keep internally optimum environmental condition. Age-of-air concept allows to assess the ventilation efficiency of an agricultural facility according to estimating the ability of fresh air supply and contaminants emission using LMA and LMR. Most of these methods use a tracer gas method which has some limitations in experiment like dealing unstable and invisible gas. Therefore, the aim of this study was to develop a straightforward method to calculate age-of-air values with CFD simulation which has the advantage of saving computational time and resources and these method can solve the limitations in experiment using tracer gas method. The main idea of LMA computation is to solve the passive scalar transport equation with the assumption that the production of the time scalar throughout the room is uniform. In case of LMR calculation, the transport of the time scalar was reversed compulsively using UDF. The methodology to validate the results of this study was established by comparing with preceding research that had performed a computing LMA and LMR value by laboratory experiments and CFD simulations using tracer gas. As a result, the error was presented similarly level of results of preceding research. Some big errors could be caused by stagnated area and incongruity turbulence model. while the computational time was reduced to almost one fourth of that by preceding research.