${eta}$-Ionone의 인체 비소폐암세포 A-549 cells (human non-small lung cancer A-549 cell)에 대한 anti-proliferative effect에 관한 연구를 수행하였다. A-549 cell에 다양한 농도의 ${eta}$-ionone (1, 5, 10, and 15 ${mu}M$)을 2, 4, 6일 간 처리하고, 배양 2일에 A-549 cell의 생육억제와 관련되는 biochemical marker를 측정하였다. ${eta}$-Ionone은 A-549 cell 생육을 dose와 time 의존적으로 저해하였다. ${eta}$-Ionone 배양 2일의 $IC_{50}$은 5.0 ${mu}g/ml$이었다. ${eta}$-Ionone은 농도 의존적으로 apoptosis를 유도하였다. ${eta}$-Ionone은 p53, p21 및 Bax protein 수준을 증가시켰으나, Bcl-2 protein 발현은 억제시켰다. ${eta}$-ionone은 cytosol cytochrome c 함량을 증가시켰고, caspase-9과 caspase-3 효소 활성증가를 유도하였다. 또한, ${eta}$-ionone은 $cPLA_2$와 COX-2 protein level을 감소시켰다. 이와 같은 결과는 ${eta}$-ionone의 A-549 cell에 대한 생육억제효과는 Bax와 Bcl-2 gene 발현을 reciprocal regulation하여 유도한 apoptosis와 $cPLA_2$ 및 COX-2 protein 발현 억제에 기인함을 의미한다.
The anti-proliferative activity of ${eta}$-ionone was investigated on human non-small lung cancer A-549 cells (designated A-549 cells). A-549 cells were treated with various concentrations of ${eta}$-ionone (1, 5, 10, and 15 ${mu}M$) for two, four, and six days. Biochemical markers related to the growth inhibition of A-549 cells by ${eta}$-ionone were measured at the second day of incubation. ${eta}$-Ionone inhibited the growth of A-549 cells by dose-and time-dependent manners, resulting in an $IC_{50}$ of 5.0 ${mu}g/ml$ at the second day of incubation. ${eta}$-Ionone induced apoptosis by a dose-dependent manner. ${eta}$-Ionone increased levels of p53, p21, and Bax proteins, but suppressed expression of the Bcl-2 protein. Similarly, ${eta}$-ionone enhanced cytochrome c release from the mitochondria to the cytosol, and induced activation of caspase-9 and -3. Additionally, ${eta}$-ion-one reduced $cPLA_2$ and COX-2 protein levels. These results suggest that the ${eta}$-ionone inhibits the proliferation of A-549 cells through reciprocal regulation of Bax and Bcl-2 gene expression and suppression of $cPLA_2$ and COX-2 protein expressions.
