The purpose of this study examined the effects of sexual autonomy, sexual assertiveness, sexual subjectivity on sexual behavior among university students. The data was collected from 226 university students and analyzed with t-test and multiple regression using SPSS 21.0 program. The significant factors influencing sexual behavior were sexual attitude, sexual assertiveness, sexual autonomy and sexual subjectivity. And these factors explained 35.7% of the variance in sexual behavior. In conclusion, the results from this study indicated a need to develop the sex education program to improve sexual health among university students.