The fungal endophyte EML-CM25 was isolated in Korea from surface sterilized Chinese maple leaf tissues. Internal transcribed spacer sequence analysis indicated that the isolate showed 99% sequence similarity with Paraconiothyrium brasiliense, a finding that was confirmed by morphological analyses. The fungal colonies did not express aerial hyphae. Conidiomata formation was observed in the fungus cultured on potato dextrose agar at $25^{circ}C$ for 7 days. Visible scattered black dots consisting of pycnidia were present throughout the colony. This is the first record of P. brasiliense in Korea.