Non-military researchers and civil manufacturers face several difficulties in the study of protective materials for chemical-biological defense. The performance of these materials cannot be estimated in non-governmental circles as the private use of chemical warfare agents (CWAs), such as GD (soman) and HD (mustard), by civilians is not permitted. The barrier properties of protective materials can be evaluated only in military laboratories, by the TOP 8-2-501 test method, using real CWAs. In this study, the permeation of CWA simulants such as dimethyl methyl phosphonate (DMMP), triethyl phosphate (TEP), and chloroethyl phenyl sulfide (CEPS) was investigated by the chemical permeation test method. Moreover, permeation of the CWA simulants through different samples of protective materials could be estimated by the proposed cumulative permeation measurements, using an absorbent tube.