PURPOSE: This study was to investigate the effect of direction of gliding in the tibiofibular (TF) joint on angle of active ankle dorsiflexion (AADF). METHODS: Fifteen subjects with no medical history of lower extremities were recruited in this study. The digital dynamometer was used to maintain the same gliding force in each condition. The angle of AADF was measured by using the electronic goniometer. Subjects were instructed to perform the AADF in three trials during the each gliding in the TF joint by the examiner. The conditions were no gliding and four directions of gliding in the proximal (anterior-superior: A-S vs posterior-inferior: P-I) and distal (posterior-superior: P-S vs. anterior-inferior: A-I) TF joint. A repeated measured ANOVA was used to compare angle of the AADF in each TF joint. The paired-sample t tests with Bonferroni correction were used in order to Post hoc pair-wise comparisons. The significant level was set at 0.016 (0.05/3). RESULTS: In distal TF joint, the angle of AADF in the A-I direction of gliding was significantly lower than those in no gliding and P-S direction of gliding (p<0.01). In proximal TF joint, the angle of AADF was significantly lower than those in no gliding and A-S direction of gliding (p<0.01). Although there was no significant differences, angle of AADF were largest in the P-S direction of gliding among four conditions. CONCLUSION: These findings suggest that gliding technique of TF joint would be required to improve the angle of AADF in subjects with limitation of ankle dorsiflexion.