Static loading tests were carried out in this study to investigate the effect of bar cutoff on the resistance of RC beam-column sub-assemblages under column loss. Two specimens were designed with continuous main reinforcement. Four others were designed with different types of bar cutoff in the mid-span and/or the beam-end regions. Compressive arch and tensile catenary responses of the specimens under gravitational loading were compared. Test results indicated that those specimens with approximately equal moment strength at the beam ends had similar peak loading resistance in the compressive arch phase but varied resistance degradation in the transition phase because of bar cutoff. The compressive bars terminated at one-third span could help to mitigate the degradation although they had minor contribution to the catenary action. Among those cutoff patterns, the K-type cutoff presented the best strength enhancement. It revealed that it is better to extend the steel bars beyond the mid-span before cutoff for the two-span beams bridging over a column vulnerable to sudden failure. For general cutoff patterns dominated by gravitational and seismic designs, they may be appropriately modified to minimize the influence of bar cutoff on the progressive collapse resistance.