The purpose of this study was to evaluate the cyclic behavior of steel column-tree moment connections used in steel moment resisting frames. These connections are composed of shop-welded stub beam-to-column connection and field bolted beam-to-beam splice. In this study, the effects of beam splice length on the seismic performance of column-tree connections were experimentally investigated. The change of the beam splice location alters the bending moment and shear force at the splice, and this may affect the seismic performance of column-tree connections. Three full-scale test specimens of column-tree connections with the splice lengths of 900 mm, 1,100 mm, and 1,300 mm were fabricated and tested. The splice lengths were roughly 1/6, 1/7, 1/8 of the beam span length of 7,500 mm, respectively. The test results showed that all the specimens successfully developed ductile behavior without brittle fracture until 5% radians story drift angle. The maximum moment resisting capacity of the specimens showed little differences. The specimen with the splice length of 1,300 mm showed better bolt slip resistance than the other specimens due to the smallest bending moment at the beam splice.