In this study, the insecticidal effect of the ethanol extract of Areca catechu against the house dust mite, Dermtophagoides pteronysinus, was examined. Ethanolic Areca catechu extract was directly applied at different concentrations (1.0, 0.5, 0.25, 0.125, 0.0625 mg/$40{mu}l$) and then allowed an exposure of 24 hours. The 1.0 mg/$40{mu}l$ and 0.5 mg/$40{mu}l$ concentrations of Areca catechu ethanol extract produced mortality rates of 100% and 98%, respectively, against house dust mite, Dermtophagoides pteronysinus. Areca catechu ethanol extract at a concentration of 0.125 mg/$40{mu}l$ produced a 50% insecticidal effect. These results proved the insecticidal effect of Areca catechu ethanol extract against house dust mite, Dermtophagoides pteronysinus. To check for the presence of the recognized insecticidal arecoline and monoterpene compounds in Areca catechu, gas chromatography-mass spectrometry and thermal desorption-gas chromatography/mass spectrometry were performed. As a result, arecoline and ten monoterpene compounds were identified in Areca catechu.