Purpose: The purpose of this study was to determine the dimensional stability of three rubber impression materials (polysulfide, polyvinylsiloxane, polyether) in an iodophor disinfectant. Materials and Methods: Seventy-two impressions were made with each material and divided into control, 10-minute, and 30-minute immersion groups using an iodophor as the disinfectant. A microscope was used to measure dimensional changes in the stone casts made from each group of impressions, and the percent change was analyzed using a two-way ANOVA (P = 0.05). Results: All dimensional changes after iodophor disinfection were small in 10 minute groups, however, showed significantly increased in 30 minute groups (P < 0.05). Conclusion: Clinically, it can be recommended to use iodophor disinfectant for rubber materials within 10 minute.