Objectives The purpose of this study was to examine the effects of positive and negative emotion on the quality of life(QoL) in each Sasang contitution, using Positive Affect and Negative Affect Schedule(PANAS) and Short Form-12 Health Survey Questionnaire(SF-12) questionnaire. Methods A total of 1,123 participants completed the Korean version of the PANAS and SF-12 questionnaire. The participants were classified into one Sasang constitution by QSCC II. The effects of emotion on the QoL were compared between the each Sasang contitution using analysis of variance(ANOVA). Results We found significant differences between constitutions in Positive Affect(PA), PA-Joy, PA-Interest and PA-Activation of PANAS. And we found significant differences between constitutions in Mental Component Summary(MCS) of SF-12. Negative correlation of less -0.4 was shown in Negative Affect(NA), NA-Afraid, NA-Upset of PANAS and MCS of SF-12. Conclusions The Soyangin has significantly higher score than Soeumin in PA, PA-Joy, PA-Interest and PA-Activation of PANAS as previous studies. This study also shows the correlation between negative emotion and decline in the quality of mental status.