In this study, the structural performance of a seven span masonry arch bridge was evaluated. Investigations were performed on Aspendos (Belkis) Masonry Arch Bridge which was located on road of Aspendos Acropolis City in Antalya, Turkey. The old bridge was constructed in the early of fourth century AD, but it was exposed to the earthquakes in this region and the overloading by the river water. The old bridge was severely damaged and collapsed by probably an earthquake many years ago and a new bridge was then reconstructed on the remains of this old bridge by Seljuk in the 13th century. The bridge has also been affected from overflowing especially in the spring of each year, so some protective measures should be taken for this monumental bridge. Therefore, the structural performance under these loading has to be known. For this purpose, an initial finite element model was developed for the bridge and it was calibrated according to ambient vibration test results. After that, it was analyzed for different load cases such as dead, live, earthquake and overflow. Three load combinations were taken into account by deriving from these load cases. The displacements and the stresses for these combination cases were attained and compared with each other. The structural performance of Aspendos Masonry Arch Bridge was determined by considering the demand-capacity ratio for the tensile stress of the mortar used in Aspendos Masonry Arch Bridge. After these investigations, some concluding remarks and offers were presented at the end of this study.