In this study, the correlation between the uneven dyeing and absorption of mulberry fibers was investigated by an effective vertical strip test. The purpose of this study was to determine the effect of uneven dyeing on the difference between the absorption of mulberry fibers. The samples, tape yarns with a width of 1.5 mm, were subjected to the vertical strip test; subsequently, they were subjected to statistical analysis to estimate the evaluation of the test method. The morphology and chemical composition of the samples were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The statistical results confirmed that a strong correlation existed between the absorption and uneven dyeing of mulberry fibers ($F_o=1.849$, P=0.0012).