Recently, a demand for precision 5-axis machine tools is significantly increasing, and the maintenance of machine tool accuracy becomes more important. it is very difficult to evaluate to accuracy of 5-axis M/C in the production site since it needs expensive measuring equipment and skilled engineer. On the other hand, evaluation items of 5-axis M/C are not systematically organized in the existing KS and ISO standards. In this study, the evaluation items for 5-axis M/C were derived systematically and a test workpiece was developed to evaluate the machine tool accuracy more easily. The error sources of machine tool can be estimated by machining and measuring of the test workpiece. The correlation between the machine tool accuracy and the accuracy of machined test workpiece was analyzed. As a result, the accuracy of machined test workpiece represented the accuracy of machine tool and the error sources very effectively.