Growing concerns about environmental pollution have led to an increase in the demand for compressed natural gas (CNG) vehicles in recent years. CNG vehicles are equipped with a cylinder valve installed in a high-pressure vessel to control the CNG flow. The cylinder valve must meet high quality safety standards because the pressure vessel stores high-pressure CNG. Therefore, safety evaluation of the cylinder valve is necessary to ensure the safety of CNG vehicles. In this study, fluid-structure interaction analysis for the structural integrity of the cylinder valve were conducted using a commercial finite element analysis code(ANSYS WORKBENCH V14). The CFD analysis was performed using a steady-state technique according to the inlet and outlet pressures in order to predict the pressure distribution. Structural analysis was performed by a static structure technique at the maximum working pressure to evaluate the structural integrity of the cylinder valve. From the results, the safety factor of the valve component is between 1.57 and 21.5.