Micro/nano-injection molding is one of the main processing techniques for polymer micro-fabrication. Most of the difficulties encountered in polymer micro-molding are caused by the demolding, rather than the filling of molds. Therefore, studying the demolding process is vitally important for manufacturing polymer replicas. The most important parameters are the thermal stress, friction and adhesion forces, and mechanical strength of the resist. In this research, we determinedthe effects of the processing conditions on the ejection force for cases involving two common thermoplastic polymers. The results showed that the processing conditions noticeably influenced the ejection force.