To improve the performance of clutch actuator of clutch-by-wire system for commercial vehicles, it is necessary to understand the driving characteristics of the system. To explain and predict the effects of driving characteristics on clutch characteristics, AMESim software is used. The simulation model of clutch-by-wire system is developed in the AMESim environments under the geometrical dimensions and driving mechanisms of the clutch-by-wire system, such as the rotation speed of the DC motor, the gear ratio of the reducer, the design parameters of the release fork, the coefficient of the clutch diaphragm spring, and so on. The results show that the theoretical analysis of the clutch-by-wire system for commercial vehicles using the AMESim software find out the driving characteristics of the clutch actuator, and predict the performance characteristics of the clutch-by-wire system.