Purpose: The purpose of this study was to identify quality of sleep and health-promoting behaviors in rotating-shift nurses and to explore the influence of health promoting behaviors on quality of sleep. Method: Participants were 161 staff nurses working in an irregular three shift system in one of three general hospitals located in Kyungpook province. Data were collected using the Pittsburgh Sleep Quality Index and the Health Promoting Lifestyle Profile-IIand were analyzed using hierarchical multiple regression analysis. Results: The majority of the participants had very poor quality of sleep and performed a low level of health promoting behaviors. In terms of health promoting behaviors, nutrition and stress management significantly influenced quality of sleep of these nurses. Conclusion: Results indicate the importance of encouraging rotating-shift nurses to enhance their health promoting behaviors in order to improve quality of sleep.