본 연구에서는 백합과에 속하는 덩굴성 관목인 청미래덩굴 70% 에탄올 추출물과 열수 추출물의 항산화 및 항당뇨에 대한 활성을 연구하였다. 총 폴리페놀 함량 과 플라보노이드 함량 측정, 전자공여능, ABTs 소거능, SOD 유사활성능, xanthine oxidase 저해활성, ACE 저해활성, TBARs 측정, PF값 측정 및 ${alpha}$-glucosidase 와 ${alpha}$-amylase 의 저해활성을 측정하였다. 그 결과 총 폴리페놀 함량과 플라보노이드 함량은 에탄올 추출물에 함유량이 더 많았으며, 전자공여능, ABTs 소거능, SOD 유사활성능, xanthine oxidase 저해활성, ACE 저해활성, TBARs 측정, PF값 측정 결과 모두 청미래 덩굴 열수 추출물보다 에탄올 추출물의 활성이 우수하였다. 또한 ${alpha}$-glucosidase 와 ${alpha}$-amylase 의 저해활성을 측정한 결과 에탄올 추출물의 활성이 열수 추출물보다 우수하였다. 이상의 검증 결과를 종합하여 볼 때 청미래 덩굴의 에탄올 추출물의 항산화 및 항 당뇨 활성이 우수하였고, 차후 항산화 및 항 당뇨 예방물질 소재로서의 활용이 가능할 것으로 판단된다.
This study was carried out to analyze the effects of water and 70% ethanol extract on the antioxidative and antidiabetic activities of Smilax china L., a vine shrub belonging to the lily family. The activities of the extracts were measured based on the total phenolic and flavonoid contents and through on the results of the antioxidant tests, such as the electron-donating ability, ABTs radical scavenging activities, SOD-like activity, xanthine oxidase inhibition effect, antioxidant protection factor (PF), TBARs content and ACE inhibition activity, and ${alpha}$-glucosidase, and ${alpha}$-amylase inhibition activity. The resulting total phenolic and flavonoid contents of the 70% ethanol extract from S. china L. were greater than those of the water extract from S. china L. With regard to the results of the antioxidant tests, such as the electron-donating ability, ABTs radical scavenging activity, SOD-like activity, xanthine oxidase inhibition effect, antioxidant protection factor (PF), and TBARs content, those from the 70% ethanol extract from S. china L. were greater than those from the water extract from S. china L. Also, with regard to the ACE inhibition effect and ${alpha}$-glucosidase and ${alpha}$-amylase inhibition, those from the 70% ethanol extract from S. china L. were greater than those from the water extract from S. china L. All these findings show that the 70% ethanol extract from S. china L. has greater antioxidative and antidiabetic effects and can be used as a preventive agent for oxidation and diabetes.
