An experiment to calibrate temperature sensors at cryogenic temperature has been performed. The main objective of this study was to develop a precise instrument for calibrating the temperature sensors over a temperature range of 4 K to approximately room temperature without liquid helium. The instrument consists of radiation shields, a sensor block, an electric heater, a cryocooler and a vacuum chamber. In a vacuum chamber, the cold head of the cryocooler is thermally anchored to the sensor block to bring the apparatus to a desired temperature. An electric heater is placed at the second stage of the cryocooler to control the temperature of the sensor block. The entire apparatus is covered by radiation shields and wrapped in multi-layer insulation to minimize thermal radiation in a vacuum chamber. The dimensions of components including instrumental wires are optimized to reduce total heat invasion from room temperature into cryogenic temperature. The vacuum chamber is pumped down and cooled to cryogenic temperature by a cryocooler. The resistance of each temperature sensor is measured at steady state as well as cooling down and warming up cycles, and the performance of calibration is discussed with respect to the sensitivity and resolution.