In this study, we investigated whether A. distichum decreases the production of inflammatory mediators through downregulation of the NF-${kappa}B$ and ERK pathway. Our data indicated that A. distichum leaf inhibits the overexpression of iNOS in protein and mRNA levels, and subsequently blocked LPS-mediated NO overproduction in RAW264.7 cells. A. distichum leaf inhibited $I{kappa}B-{alpha}$ degradation and p65 nuclear translocation, and subsequently suppressed transcriptional activity of NF-${kappa}B$ in LPS-stimulated RAW264.7 cells. In addition, A. distichum leaf suppressed LPS-induced ERK1/2 activation by decreasing phosphorylation of ERK1/2. These findings suggest that A. distichum leaf shows anti-inflammatory activities through suppressing ERK-mediated NF-${kappa}B$ activation in mouse macrophage.