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Effects of Quercetin and Genistein on Boar Sperm Characteristics and Porcine IVF Embyo Developments
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  • Effects of Quercetin and Genistein on Boar Sperm Characteristics and Porcine IVF Embyo Developments
  • Effects of Quercetin and Genistein on Boar Sperm Characteristics and Porcine IVF Embyo Developments
저자명
Kim. Tae-Hee,Yuh. In-Suh,Park. In-Chul,Cheong. Hee-Tae,Kim. Jong-Taek,Park. Choon-Keun,Yang. Boo-Keun
간행물명
韓國受精卵移植學會誌
권/호정보
2014년|29권 2호|pp.141-148 (8 pages)
발행정보
한국수정란이식학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Quercetin and genistein, plentifully present in fruits and vegetables, are flavonoid family members that have antioxidative function and plant-derived phytoestrogen activity. The antioxidative effects of quercetin and genistein on boar sperm characteristics and in vitro development of IVF embryo were investigated. The sperm motility was increased by addition of genistein $50{mu}M$ for 6 hr incubation compared to control (p<0.05). The sperm viability was increased by addition of quercetin 1 and $50{mu}M$ and genestein 1 and $50{mu}M$ for 3 hr incubation. In addition, the sperm viability seemed to be increased dose-dependantly by addition of quercetin or genistein 1 and $50{mu}M$, respectively (p<0.05). The membrane integrities were not increased by quercetin or genistein treatments for 3 hr or 6 hr incubation period except for quercetin $1{mu}M$ for 3 hr incubation. In mitochondrial activities, addition of quercetin $50{mu}M$ for 6 hr incubation increased mitochondrial activity but decreased at $100{mu}M$ concentration compared with control (p<0.05). When porcine IVF embryos were cultured in PZM-3 medium supplemented with low concentrations of quercetin ($1{sim}10{mu}M$), the developmental rates to morula and blastocyst increased but significantly decreased at high concentrations of quercetin ($25{sim}50{mu}M$). The highest developmental rate to blastocysts among all concentrations of quercetin was shown at quercetin $10{mu}M$ (p<0.05). The developmental rates to morula or blastocysts at low ($0.01{sim}1{mu}M$) and high ($5{sim}10{mu}M$) concentrations of genistein were not significantly different among all treatment group and genistein did not affect on IVF embryo development. These results suggest that quercetin and genistein seem to have positive effects at certain concentrations on sperm characteristics such as motility, viability and mitochondrial activity. In addition, low concentrations of quercetin (1, 5 and $10{mu}M$) in this experiment, seem to have beneficial effect on porcine IVF embryo development but genistein did not affect on it at all given concentrations ($0.01{sim}10{mu}M$).