This study verified the effect of summer flight ventilation developed in a previous study based on wearing comfort evaluation. Seven healthy males in their twenties volunteered for this experiment conducted in aclimatic chamber. The experiment consisted of three consecutive periods of rest (20 minutes), running on a treadmill (10 minutes) and recovery (20 minutes). A comparative evaluation was conducted on the general flight suit which had no ventilation holes and summer flight suit that use subjective satisfaction measures and objective measures. The subjective satisfaction was evaluated according to the criteria of temperature sensation, wet sensation, thermal comfort and fatigue sensation. The objective satisfaction was measured by skin temperature, microclimate (temperature and humidity), sweat rate and thermography. The comparative wearing evaluation identified the summer flight suit decreased the temperature between skin and suit by $0.42^{circ}C$ (upper arm), $0.9^{circ}C$ (calf) and the skin temperature by $0.3^{circ}C$ (shoulder), $0.4^{circ}C$ (upper arm), $0.5^{circ}C$ (calf) as compared to the general flight suit. The humidity inside the summer flight suit decreased at head (7.73%), shoulder (5.86%), upper arm (5.26%), and calf (8.73%) compared to the one inside the general flight suit. Thermography showed that the air flowed through ventilation holes (neck and armpit). The design of ventilation holes applied to the summer flight suit can be applicable to overall clothing that requires thermal comfort such as dust-free garments, mechanical clothing and combat uniforms.