Navy forces of ROK asked for more than 90% operational availability in the requirement document of combat ship. This study proposes the evaluation methodology of operational availability with the evaluation process, calculation formula, analysis of operational test data. As the case study, the developed methodology is proved to apply for 00 batch-I naval ship using the data to be acquired during the operational test period. The operational availability by test data was 90.03%, and it was satisfied with objective value 90%. The paper will contribute not only to establish the evaluation methodology of operational availability for combat ship but also other general weapon system.