Due to the sea-crossing bridge span is generally large and main pier pile foundations are located in deep water and carry large vertical load, sea-crossing bridge main pier pile foundations bearing mechanism and load deformation characteristics are still vague. Authors studied the vertical bearing properties of sea-crossing bridge main pier pile foundations through pilot load tests. Large tonnage load test of Qingdao Bay Bridge main pier pile program is designed by using per-stressed technique to optimize the design of anchor pile reaction beam system. Test results show that the design is feasible and effective. This method can directly test bearing capacity of main pier pile foundations, and analysis bearing behaviors from test results of sensors which embedded in the pile. Through test study the vertical bearing properties of main pier pile foundation and compared with the generally short pile, author summarized the main pier pile foundations vertical bearing capacity and the main problem of design and construction which need to pay attention, and provide a reliable basis and experience for sea-crossing bridge main pier pile foundations design and construction.