This paper proposes a new assessment and reliability validation method of Lane Departure Assistance System based on DGPS-GIS by measuring lanes with camera vision. Assessment of lane departure is performed with yaw speed measurement and determination method for false alarm of ISO 17361 and performance validation is executed after generating departure warning boundary line by considering deviation error of LDAS using DGPS. Distance between the wheel and the lane is obtained through line abstraction using Hough transformation of the lane image with camera vision. Evaluation validation is obtained by comparing this value with the distance obtained with LDAS. The experimental result shows that the error of the extracted distance of the LDAS is within 5 cm. Also it proves performance of LDAS based on DGPS-GIS and assures effectiveness of the proposed validation method for system reliability using camera vision.