PURPOSE: The purpose of this study was to investigate any changes in abdominal muscle activity after curl-up and jack-knife exercisse using a sling and swiss-ball. METHODS: Sixteen asymptomatic subjects were randomly assigned into either a sling exercise group (SG) or swiss-ball exercise group (SBG). Subjects performed curl-up and jack-knife exercise using sling and swiss ball. Activity of abdominal muscles (rectus abdominis and external oblique) was assessed using surface electromyography (EMG) and normalized maximal voluntary isometric contraction (MVIC) before and after exercises. The significance of differences between the SG and the SBG was evaluated by analysis of covariance (ANCOVA). RESULTS: There was an increase in activity of all abdominal muscles after the curl-up and jack-knife exercises for 6 weeks. However, there was not a significant difference between the SG and the SBG after curl-up exercise (p>.05). There was also not a significant difference between the SG and the SBG after Jack-knife exercise (p>.05) except for the right external oblique muscle (p<.05). CONCLUSION: Exercises on an unstable surface using a sling and swiss ball are effective ways of abdominal muscle exercise.