The purpose of this study was to analyze the changes in muscle fatigue by using electromyography (EMG) measurements of the median frequency of shoulder muscles of patients who underwent rotator cuff repair, and to provide basic data for efficient exercise methods during rehabilitation after the repair. In the study, 24 male and 8 female patients who underwent rotator cuff repair performed continuous passive shoulder joint motion for 3 weeks and the median frequency of their shoulder muscles before and after the repair were compared using measurements by EMG. The infraspinatus, supraspinatus, and serratus anterior muscles showed significant differences in the changes in the median frequency before and after the repair. Significant differences were also found in the median frequency of the three muscles both before and after the repair. Additionally, the middle deltoid muscle had a lower median frequency than the supraspinatus muscle before the repair and the infraspinatus muscle had a lower median frequency than the middle deltoid muscle after the repair. It is expected that these results will serve as important data in developing rehabilitation exercise programs for the infraspinatus, supraspinatus, and serratus anterior muscles by compensating the middle deltoid muscle with the lowest muscle fatigue after the rotator cuff repair erase.