Phytoplankton species composition and ecological index (diversity, evenness, richness and dominance) were analysed from April 2013 to March 2014 at 10 stations of cupped oyster, Crassostrea gigas culture area in Iwon coast, Korea. Seasonal and positional variation of phytoplankton standing crops, biomass, dominant species and water quality were distinctively different according to occasionally inflow of Iwon dam reservoired water. The composition of phytoplankton species were Bacillariophyceae 98, Dinophycease 22, Chlorophycease 13, Cyanophyceae 8, Silicofalgellate 4, Euglenophyceae 2, Cryptophyceae 1 species. The most dominant species was Bacillariophyceae as 64.0%. The highest biomass of phytoplankton recorded in September as $40,910{ imes}10^3$ cell/L at the station 1, near from inland water inflow area. Ecological indices (diversity, richness, evenness, and dominance index), used for structural change of phytoplankton community and water quality (temperature, dissolved oxygen, salinity) showed difference of spatiotemporal property also.