Imha Reservoir is connected to Andong Reservoir via a diversion tunnel allowing water to pass between. The diversion tunnel is equipped with screens to exclude exotic largemouth bass due to their predatory impacts on prey assemblages resulting in a degradation of species richness of local fish fauna and extinction of local fish populations in Korea. Flow pattern changes resulting from the fish screens and trash racks were investigated using a computational fluid dynamics (CFD) model. Numerical simulations showed that the decrease in the discharge capacity of the tunnel is approximately 8.6% and the headloss coefficient for fish screen at Andong intake tower was determined to be 1.5. In order not to allow the small fishes enough to pass through the wire openings enter into Imha Reservoir through tunnel, the velocity in the tunnel should be greater than 1.48 m/s which is a critical ascending velocity of the bass. This study suggests that it can keep the velocity higher enough to exclude largemouth bass when a gate opens with the condition of 1.0m difference in water stage between two reservoirs.