Emission standards for passenger diesel engines are becoming more and more stringent. Especially, Europe started the regulation of nano-particles from 2011 with EURO 5b. The objective of this study is to investigate the effect of fuel injection strategy on combustion and nano-particle emission in a small diesel engine. In this study, we conducted combustion analysis and measured both the weight of PM and number of nano-particels. At first, the optimum injection timing was determined with fixed engine operating conditions, such as engine speed, load, and fuel injection quantity. After that, the injection timing was controlled, and the effect of pilot injection was investigated. The number of nano-particles increased as engine load decreases, and it increased up to 10 times depending on the change of injection timing. The weight of PM emissions was increased at low load, and the PM emissions increased with increasing the number of pilot injections.