When large penetration of the distributed generators (DGs) such as photovoltaic (PV) systems is growing up in grid system, it is important to quickly prevent islanding caused by power system fault to ensure electrical safety. We propose a novel active method for islanding prevention by harmonic injection synchronized with the exciting current harmonics of the pole transformer to avoid mutual interference between active signals. We confirm the validity of the proposed method by performing the basic tests of islanding by using a current source superimposed the harmonic active signal. Further, we carry out the simulation using PSCAD/EMTDC, and verify the fast islanding detection.